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Excision of the En/Spm transposable element of Zea mays requires two element‐encoded proteins.
Author(s) -
Frey M.,
Reinecke J.,
Grant S.,
Saedler H.,
Gierl A.
Publication year - 1990
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1990.tb07625.x
Subject(s) - biology , transposable element , zea mays , dna transposable elements , element (criminal law) , genetics , gene , genome , agronomy , law , political science
An excision assay system for En/Spm was developed in transgenic tobacco. The characteristics of excision and integration are similar to the natural system of Zea mays. In this transgenic model system two En/Spm encoded trans‐acting functions, TNPA and TNPD, are required for excision. A biochemical model for transposition is proposed that might also be applicable to other transposable elements.