Premium
The nematode spliced leader RNA participates in trans‐splicing as an Sm snRNP.
Author(s) -
Maroney P. A.,
Han G. J.,
Denker J. A.,
Nilsen T. W.
Publication year - 1990
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1990.tb07578.x
Subject(s) - biology , snrnp , rna splicing , trans splicing , nematode , rna , ribonucleoprotein , genetics , rna binding protein , microbiology and biotechnology , gene , ecology
The trans‐spliced leader RNA (SL RNA) of nematodes resembles U snRNAs both in cap structure and in the presence of a consensus Sm binding site. We show here that synthetic SL RNA, synthesized by in vitro transcription, is efficiently used as a spliced leader donor in trans‐splicing reactions catalyzed by a cell free extract prepared from developing embryos of the parasitic nematode, Ascaris lumbricoides. Efficient utilization of synthetic SL RNA requires a functional Sm binding site. Mutations within the Sm binding sequence that prevent immunoprecipitation by Sm antisera and prevent cap trimethylation abolish trans‐splicing. The effect on trans‐splicing is not due to undermethylation of the cap structure.