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Identification of a potential RNA intermediate for transposition of the LINE‐like element I factor in Drosophila melanogaster.
Author(s) -
Chaboissier M. C.,
Busseau I.,
Prosser J.,
Finnegan D. J.,
Bucheton A.
Publication year - 1990
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1990.tb07566.x
Subject(s) - pascal (unit) , drosophila melanogaster , biology , genetics , computer science , gene , programming language
The I factor, a transposable element related to mammalian LINEs, controls the I‐R system of hybrid dysgenesis in Drosophila melanogaster. It transposes at high frequency in the germ‐line of the female progeny of crosses between females of the reactive class of strains and males of the inducer class. The structure and DNA sequence of the I factor suggest that it transposes by reverse transcription of an RNA intermediate. Northern blot and S1 mapping experiments show that a full‐length RNA of the I factor is synthesized specifically in the conditions of which I factors transpose. This RNA has all characteristics of a transposition intermediate. It is only found in the ovaries of dysgenic females suggesting that I factor activity is restricted to this tissue because of regulation at the level of the initiation of transcription or RNA stability.