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T cell precursor migration towards beta 2‐microglobulin is involved in thymus colonization of chicken embryos.
Author(s) -
Du D.,
Kaufman J.,
Salomonsen J.,
Skjoedt K.,
Vainio O.,
Thiery J. P.,
Imhof B. A.
Publication year - 1990
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1990.tb07531.x
Subject(s) - biology , library science , immunology , computer science
beta 2‐microglobulin (beta 2m) attracts hemopoietic precursors from chicken bone marrow cells in vitro. The cell population responding to beta 2m increases during the second period of thymus colonization, which takes place at days 12‐14 of incubation. The precursors from 13.5 day old embryos were isolated after migration towards beta 2m in vitro and shown to be able to colonize a 13 day old thymus in ovo, where they subsequently acquire thymocyte markers. In contrast these beta 2m responsive precursors did not colonize embryonic bursa, i.e. differentiate into B lymphocytes. During chicken embryogenesis, peaks of beta 2m transcripts and of free beta 2m synthesis can only be detected in the thymus. The peak of free beta 2m synthesis in the thymus and the increase of beta 2m responding bone marrow cells both occur concomitantly with the second wave of thymus colonization in chicken embryo, facts which suggest that beta 2m mediated chemotaxis is involved in the second wave.