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Signals at the bacteriophage phi 29 DNA replication origins required for protein p6 binding and activity.
Author(s) -
Serrano M.,
Gutiérrez J.,
Prieto I.,
Hermoso J. M.,
Salas M.
Publication year - 1989
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1989.tb03584.x
Subject(s) - biology , bacteriophage , microbiology and biotechnology , humanities , genetics , art , gene , escherichia coli
Protein p6 of Bacillus subtilis phage phi 29 binds specifically to the ends of the viral DNA that contain the replication origins, giving rise to a nucleoprotein structure. DNA regions recognized by protein p6 have been mapped by deletion analysis and DNase I footprinting. Main protein p6‐recognition signals have been located between nucleotides 62 and 125 at the right phi 29 DNA end and between nucleotides 46 and 68 at the left end. In addition, recognition signals are also present at other sites within 200‐300 bp at each phi 29 DNA end. Protein p6 does not seem to recognize a specific sequence in the DNA, but rather a structural feature, which could be bendability. The formation of the protein p6‐DNA nucleoprotein complex is likely to be the structural basis for the protein p6 activity in the initiation of replication.