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Directionality of DnaA protein/DNA interaction. Active orientation of the DnaA protein/dnaA box complex in transcription termination.
Author(s) -
Schaefer C.,
Messer W.
Publication year - 1989
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1989.tb03545.x
Subject(s) - dnaa , transcription (linguistics) , biology , physics , dna , microbiology and biotechnology , dna binding protein , genetics , transcription factor , gene , philosophy , linguistics
The complex of DnaA protein with its 9 bp consensus binding site, the dnaA box 5′‐TT(A/T)T(A/C)CA(A/C)A, blocks transcribing RNA polymerase. In a model system, the rate of transcription was monitored distal to the dnaA box 5′‐TTTTCCACA by the expression of a reporter gene. DnaA‐dependent transcription termination occurred irrespective of whether the dnaA box region was or was not translated. Only the dnaA box orientation 5′‐TTTTCCACA on the non‐coding strand, but not the reverse orientation, was active in termination. This suggests that DnaA protein contacts only one strand of the DNA duplex. Oligonucleotide‐directed mutation of a dnaA box present within the dnaA coding region resulted in increased expression of dnaA. This demonstrates that DnaA protein‐directed transcription termination is an element of the autoregulation of the dnaA gene.