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A novel spatial transcription pattern associated with the segmentation gene, giant, of Drosophila.
Author(s) -
Mohler J.,
Eldon E. D.,
Pirrotta V.
Publication year - 1989
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1989.tb03538.x
Subject(s) - biology , genetics , drosophila melanogaster , gene , transcription (linguistics) , drosophila (subgenus) , segmentation , computational biology , evolutionary biology , microbiology and biotechnology , artificial intelligence , philosophy , linguistics , computer science
The segmentation gene, giant, is located in 3A1 within a cloned chromosome region surrounding the zeste locus. Rearrangement breakpoints associated with giant mutations were localized on the genomic clone map, and nearby transcription units were identified. One transcription unit is active during early embryogenesis and its transcripts are spatially localized from blastoderm into extended germband stages, consistent with expected expression patterns predicted by the ‘gap’ phenotype of giant mutants. Germ line transformation experiments using a 10‐kb DNA fragment containing this transcription unit gave complete rescue of the abdominal giant defect but only partial correction of the head defect. The effect of mutations in three other gap loci, Kr, kni and hb, were also analyzed.