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Identification and electron microscopic analysis of a chaperonin oligomer from Neurospora crassa mitochondria.
Author(s) -
Hutchinson E. G.,
Tichelaar W.,
Hofhaus G.,
Weiss H.,
Leonard K. R.
Publication year - 1989
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1989.tb03532.x
Subject(s) - neurospora crassa , biology , oligomer , neurospora , crassa , chaperonin , mitochondrion , identification (biology) , microbiology and biotechnology , biochemistry , genetics , protein folding , botany , gene , mutant , chemistry , organic chemistry
A 7‐fold symmetric particle has been identified in Neurospora crassa which is most probably the mitochondrial chaperonin. The particle, about 12 nm in diameter, appears in preparations of cytochrome reductase, and is shown to contain a 60 kd protein which cross‐reacts with anti‐GroEL antibodies. Results of STEM mass measurement suggest that the particle is composed of 14 subunits. A preliminary interpretation of the structure of the particle based on electron microscopy is given. Its quaternary structure and molecular weight are similar to those of the recently discovered family of particles called chaperonins, found in bacteria, chloroplasts and mitochondria.