Premium
Rearrangement between ornithine decarboxylase and the switch region of the gamma 1 immunoglobulin gene in alpha‐difluoromethylornithine resistant mouse myeloma cells.
Author(s) -
Katz A.,
Kahana C.
Publication year - 1989
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1989.tb03487.x
Subject(s) - biology , ornithine decarboxylase , microbiology and biotechnology , antibody , immunoglobulin heavy chain , ornithine decarboxylase antizyme , alpha (finance) , immunoglobulin gene , immunoglobulin g , gene , enzyme , biochemistry , genetics , medicine , construct validity , nursing , patient satisfaction
We demonstrate here that the functional ornithine decarboxylase (ODC) gene of alpha‐difluoromethylornithine (alpha‐DFMO, a suicide inhibitor of ODC) resistant mouse myeloma 653‐1 cells has been rearranged with the immunoglobulin heavy chain locus in a c‐myc like manner. Structural analysis of a molecular clone representing this gene revealed that it is joined in a head to head configuration to the switch region of the gamma 1 immunoglobulin gene. Comparison of this rearranged ODC gene to a germline ODC gene isolated from mouse liver DNA revealed identity in the region downstream to the breakpoint which was mapped to position −1371 +/‐ 1 relative to the transcription initiation site (position +1). In the switch region of the gamma 1 immunoglobulin gene the breakpoint falls within a 49 bp repeat, in a sequence frequently involved in class switching. This finding further supports the notion that in B cells the immunoglobulin gene clusters are prone to random rearrangements which under selection for a tumorigenic phenotype involve oncogenes. However, as demonstrated here, employment of specific pharmacological selection can reveal rearrangements with non‐oncogenic genes.