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p21H‐ras‐induced morphological transformation and increases in c‐myc expression are independent of functional protein kinase C.
Author(s) -
Lloyd A. C.,
Paterson H. F.,
Morris J. D.,
Hall A.,
Marshall C. J.
Publication year - 1989
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1989.tb03479.x
Subject(s) - library science , classics , history , computer science
It has previously been demonstrated that efficient DNA synthesis by oncogenic p21H‐ras only occurs in the presence of insulin and is absolutely dependent on functional protein kinase C. Here we show that morphological transformation induced by oncogenic p21H‐ras does not require functional protein kinase C. The early phases of protein kinase C‐independent morphological transformation do not require de novo protein synthesis. We have also demonstrated that the introduction of p21H‐ras into quiescent Swiss 3T3 cells by scrape‐loading leads to increased levels of c‐myc mRNA similar to those seen following serum stimulation. The increases in c‐myc mRNA levels induced by p21H‐ras are also independent of functional protein kinase C. Both morphological transformation and the elevation of c‐myc mRNA levels do not require insulin. These results demonstrate that p21H‐ras is generating protein kinase C‐dependent and ‐independent signals.