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Mapping of Digitaria streak virus transcripts reveals different RNA species from the same transcription unit.
Author(s) -
Accotto G. P.,
Donson J.,
Mullineaux P. M.
Publication year - 1989
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1989.tb03470.x
Subject(s) - biology , library science , streak , digitaria , botany , computer science , physics , optics
All, except 19 [corrected] bp, of the Digitaria streak virus (DSV) genome is transcribed. Two RNA transcripts (1+ and 2+) are encoded by the virion DNA strand and up to five (1‐ to 5‐) by the complementary DNA strand [corrected]. Detailed mapping of these RNAs has revealed evidence for splicing in one species (RNA 4‐), which together with its more abundant unspliced counterpart (RNA 2‐) could synthesize both a 30.5 and 41 kd polypeptide from the same transcription unit. This extensive overlapping of spliced and unspliced RNAs could indicate that the initiation and splicing of transcripts is temporally regulated. At least one transcript (RNA 1‐) may have a non‐translational role. Transcription of the DSV genome shows similarities to some animal DNA viruses, particularly the papovaviruses.