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The 5′ flanking region of the pS2 gene contains a complex enhancer region responsive to oestrogens, epidermal growth factor, a tumour promoter (TPA), the c‐Ha‐ras oncoprotein and the c‐jun protein.
Author(s) -
Nunez A. M.,
Berry M.,
Imler J. L.,
Chambon P.
Publication year - 1989
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1989.tb03443.x
Subject(s) - microbiology and biotechnology , biology , gene , physics , genetics
Expression of the pS2 gene which is transcriptionally controlled by oestrogens in the breast cancer cell line MCF‐7 is oestrogen independent in stomach mucosa. We show here that the level of MCF‐7 cell pS2 mRNA can also be increased by the tumour promoter 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA). We further demonstrate, using transient transfection assays, that the −428 to −332 5′ flanking sequence of the pS2 gene contains DNA enhancer elements responsive to oestrogens, TPA, EGF, the c‐Ha‐ras oncoprotein and the c‐jun protein.