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Electron microscopy of U1 small nuclear ribonucleoprotein particles: shape of the particle and position of the 5′ RNA terminus.
Author(s) -
Kastner B.,
Lührmann R.
Publication year - 1989
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1989.tb03374.x
Subject(s) - ribonucleoprotein , snrnp , biology , rna , warburg effect , physics , microbiology and biotechnology , biophysics , genetics , gene , cancer cell , cancer
Small nuclear ribonucleoprotein particles U1 (U1 snRNPs) from HeLa cells were purified to homogeneity and examined in the electron microscope by negative staining with the double carbon film technique. A survey of several hundred views of the U1 snRNP particles revealed a main body about 8 nm in diameter with two characteristic protuberances 4‐7 nm long and 3‐4 nm wide. Anti‐m3G antibodies were then used to define the site of the m3G base in the 5′ cap of the U1 snRNA, close to the site where base‐pairing occurs with the 5′ splice sequence of pre‐mRNA. The m3G base is located on the body of the U1 snRNP, close to one of the protuberances and approximately opposite the other.