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Epstein‐Barr virus BZLF1 trans‐activator specifically binds to a consensus AP‐1 site and is related to c‐fos.
Author(s) -
Farrell P. J.,
Rowe D. T.,
Rooney C. M.,
Kouzarides T.
Publication year - 1989
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1989.tb03356.x
Subject(s) - rowe , medical school , library science , medicine , management , medical education , computer science , economics
Two regions of the Epstein‐Barr virus BZLF1 trans‐activator protein have sequence similarity to the c‐fos protein. Part of the similarity corresponds to the region of c‐fos which is similar to the DNA binding domain of c‐jun and GCN‐4. The structure of the exon which contains this region in c‐fos and BZLF1 is also highly conserved between the two genes. Complete BZLF1 protein and a C terminal fragment were prepared either as purified fusion proteins or by in vitro translation from a BZLF1 cDNA. Gel retardation and DNase footprinting assays using these proteins show that BZLF1 is a sequence specific DNA binding protein capable of binding to a target sequence which contains a consensus AP‐1 site.