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Two different virus‐inducible elements are required for human beta‐interferon gene regulation.
Author(s) -
Fan C. M.,
Maniatis T.
Publication year - 1989
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1989.tb03353.x
Subject(s) - biology , gene , virus , interferon , irf8 , transcription (linguistics) , regulatory sequence , regulation of gene expression , response element , microbiology and biotechnology , negative regulatory element , gene expression , virology , genetics , promoter , linguistics , philosophy
We show that the human beta‐interferon gene promoter contains two different virus‐inducible regulatory elements, PRDI and PRDII (positive regulatory domains I and II). A single copy of either element alone has no discernible transcriptional activity in mouse fibroblasts. However, multiple copies of either element function as a constitutive or virus‐inducible transcription element depending on the cell line in which the sequence was tested. These results in conjunction with previous studies suggest that virus induction of the human beta‐interferon gene is achieved through cooperative interactions between two entirely distinct virus‐inducible elements. Comparison of the properties of these elements reveals that multiple copies of PRDI, but not PRDII, can be activated by three different inducers, beta‐IFN, gamma‐IFN and virus. These results suggest that the pathways of virus and interferon induction may share at least one common regulatory component.