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Fos C‐terminal mutations block down‐regulation of c‐fos transcription following serum stimulation.
Author(s) -
Wilson T.,
Treisman R.
Publication year - 1988
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1988.tb03316.x
Subject(s) - biology , mutant , transcription (linguistics) , heterologous , microbiology and biotechnology , gene , transfection , c fos , transcription factor , genetics , gene expression , linguistics , philosophy
Transient accumulation of c‐fos RNA following serum stimulation requires both a conserved 5′ regulatory element and sequences at the 3′ end of the gene. Here we show that mutations at the C terminus of Fos protein, of the type found in a virally‐transduced actively transforming Fos variant, prevent the rapid down‐regulation of c‐fos transcription that occurs following serum‐induced activation. Fos mutants that prevent down‐regulation are dominant, acting in trans to prevent down‐regulation of a co‐transfected c‐fos gene. Co‐transfection experiments suggest that this effect is mediated by multiple sequence elements in the 5′‐flanking region. Analysis of different Fos mutants showed that replacement of Fos sequences C‐terminal to amino acid 337 with heterologous polypeptide, rather than simple truncation of the protein, is required to produce mutants defective in down‐regulation. The results are discussed with reference to transformation by Fos.