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Studies of the mechanism of spontaneous germline ecotropic provirus acquisition in mice.
Author(s) -
Lock L. F.,
Keshet E.,
Gilbert D. J.,
Jenkins N. A.,
Copeland N. G.
Publication year - 1988
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1988.tb03313.x
Subject(s) - biology , provirus , mechanism (biology) , germline , genetics , virology , genome , gene , philosophy , epistemology
SWR/J–RF/J hybrid mice spontaneously acquire new germline ecotropic proviruses at high frequency. We have performed ovarian transplantation and in situ hybridization studies to delineate the mechanism and developmental stage of germline provirus acquisition. In addition, we have developed a novel, efficient and simple method to introduce single copy proviruses into the mouse germline. The results reported here have direct implications for understanding how proviruses are acquired in the germline, for using murine leukemia viruses as insertional mutagens, and for using retroviral vectors to introduce foreign genes into the mouse germline.