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Two guanosine binding sites exist in group I self‐splicing IVS RNAs.
Author(s) -
Kay P. S.,
Menzel P.,
Inoue T.
Publication year - 1988
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1988.tb03229.x
Subject(s) - guanosine , biology , rna splicing , rna , biochemistry , gene
A shortened form of the self‐splicing rRNA intervening sequence (IVS) of Tetrahymena thermophila can catalyze a transesterification reaction, termed G‐exchange, between a monomeric guanosine derivative such as GTP and the substrate GpN (where N is A, C, G or U). The reaction is specific to the two guanosines involved, providing evidence that two guanosine binding sites exist in this group I IVS RNA. One binding site accommodates a guanosine which initiates self‐splicing and the other recognizes the guanosine preceding the 3′ splice site. Previously, only one guanosine binding site was thought to be involved in the mechanism of self‐splicing. Based on the two functionally distinguishable guanosine binding sites, a new model is proposed to explain how the two independent transesterification reactions required for self‐splicing might proceed in a concerted manner.