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The human Lyt‐3 molecule requires CD8 for cell surface expression.
Author(s) -
DiSanto J. P.,
Knowles R. W.,
Flomenberg N.
Publication year - 1988
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1988.tb03221.x
Subject(s) - effector , biology , cd8 , cancer , lymphocyte , tumor infiltrating lymphocytes , monoclonal antibody , immunology , antibody , immune system , genetics
We have previously identified a monoclonal antibody, T8/2T8‐5H7, which clustered serologically with CD8 monoclonal antibodies, but lacked reactivity with L cell transfectants expressing the human CD8 molecule (Lyt‐2 homologue). Based on these observations, we postulated that T8/2T8‐5H7 might recognize the human Lyt‐3 gene product. To test this hypothesis, we have isolated a full‐length cDNA encoding the human Lyt‐3 molecule and have characterized its product in additional transfection experiments. The results of these studies indicate that the human Lyt‐3 cDNA encodes a product recognized by the antibody T8/2T8‐5H7. Interestingly, the human Lyt‐3 molecule cannot be expressed alone, but requires the human Lyt‐2 homologue for efficient cell surface expression. A heterodimer composed of the human Lyt‐2 and Lyt‐3 molecules may have importance in T cell‐target cell interactions.