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Evidence for a nuclear factor(s), IRF‐1, mediating induction and silencing properties to human IFN‐beta gene regulatory elements.
Author(s) -
Fujita T.,
Sakakibara J.,
Sudo Y.,
Miyamoto M.,
Kimura Y.,
Taniguchi T.
Publication year - 1988
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1988.tb03213.x
Subject(s) - biology , interferon regulatory factors , enhancer , gene silencing , transcription factor , irf1 , regulation of gene expression , regulatory sequence , gene , microbiology and biotechnology , genetics
Transcription of the human interferon‐beta (IFN‐beta) gene is induced by a variety of agents such as viruses, dsRNA and some cytokines. In this study, we describe a nuclear factor, termed interferon regulatory factor‐1 (IRF‐1), that is involved in the transcription of IFN‐beta and possibly other genes. We demonstrate that IRF‐1 functions in virus‐induced transcription by interacting with previously identified, IFN‐beta regulatory DNA elements. Our data suggest that IRF‐1 participates in the transient formation of an induction‐specific complex(es) with the regulatory elements. IRF‐1 may also be involved in silencing the function of the SV40 enhancer juxtaposed to the regulatory elements in uninduced cells.