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Ras (proto)oncogene induces N‐linked carbohydrate modification: temporal relationship with induction of invasive potential.
Author(s) -
Bolscher J. G.,
Bijl M. M.,
Neefjes J. J.,
Hall A.,
Smets L. A.,
Ploegh H. L.
Publication year - 1988
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1988.tb03208.x
Subject(s) - biology , carbohydrate , oncogene , genetics , microbiology and biotechnology , biochemistry , gene , cell cycle
The effect of expression of the ras oncogene on protein glycosylation was studied. VSV G‐protein and class I histocompatibility antigens were analysed to monitor ras‐mediated changes in glycosylation. Transient expression of the c‐Ha‐ras oncogene, introduced into NIH 3T3 cells by the DEAE‐dextran method, altered protein glycosylation within 25 h of transfection. The same result was obtained after dexamethasone‐induced expression of p21‐ras in stable NIH 3T3 transfectants containing either an activated Ha‐ras oncogene or a normal N‐ras proto‐oncogene under control of the glucocorticoid‐inducible MMTV promoter. The alteration of cell surface carbohydrates, induced by the ras (proto)oncogene and the subsequent acquisition of invasive potential, occurred prior to morphological transformation.