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Single genes from Agrobacterium rhizogenes influence plant development
Author(s) -
Schmülling T.,
Schell J.,
Spena A.
Publication year - 1988
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1988.tb03114.x
Subject(s) - agrobacterium , biology , gene , physics , plasmid , microbiology and biotechnology , genetics , transformation (genetics)
The combined expression of the rol A, B and C loci of Agrobacterium rhizogens Ri‐plasmids establishes, in transgenic tobacco plants, a pathological state called hairy‐root syndrome. However, when expressed separately they provoke distinct developmental abnormalities characteristic for each rol gene. Moreover, changes in their mode of expression obtained by replacing the promoters of the rol B and C genes with the cauliflower mosaic virus 35S promoter elicit new and distinct developmental patterns. These results indicate that the different rol gene products have either different targets, or have a qualitatively different effect on the same target. The target(s) must be involved in the control of plant development. Although each of the three rol genes are independently able to promote root formation in tobacco, efficient root initiation and growth is best achieved through the combined activities of more than a single rol gene. Models explaining the biological effects of A. rhizogenes ‐derived TL‐DNA genes are discussed.