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Cloning and characterization of a DNA gyrase B gene from Streptomyces sphaeroides that confers resistance to novobiocin.
Author(s) -
Thiara A. S.,
Cundliffe E.
Publication year - 1988
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1988.tb03065.x
Subject(s) - novobiocin , dna gyrase , biology , streptomyces , microbiology and biotechnology , gene , molecular cloning , bacillus subtilis , genetics , escherichia coli , peptide sequence , bacteria
A gyrB gene from Streptomyces sphaeroides, a producer of novobiocin, has been cloned in Streptomyces lividans, where it conferred resistance to novobiocin. The Streptomyces gyrB gene was sufficiently similar to a Bacillus subtilis gyrB probe to be specifically recognized during Southern analysis. Partial purification of DNA gyrase by affinity chromatography revealed the presence of two such activities (differing in their responses to novobiocin) in the clone. The product of the cloned gene, a novobiocin‐resistant DNA gyrase B subunit, was identified in vitro by coupled transcription–translation as a 79‐kd protein.