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Two different liver‐specific factors stimulate in vitro transcription from the human alpha 1‐antitrypsin promoter.
Author(s) -
Monaci P.,
Nicosia A.,
Cortese R.
Publication year - 1988
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1988.tb03047.x
Subject(s) - biology , in vitro , promoter , transcription (linguistics) , microbiology and biotechnology , transcription factor , computational biology , genetics , gene , gene expression , linguistics , philosophy
The region from −137 to −2 of the human alpha 1‐antitrypsin (alpha 1AT) promoter directs liver‐specific in vitro transcription. Two cis‐acting elements, A and B, have been identified within this segment by site‐directed mutagenesis. Competition with synthetic oligonucleotides corresponding either to the A or to the B sequence inhibits transcription from the wild‐type promoter in vitro. Cis‐linked A and B elements mediate liver‐specific transcription from a truncated HSV‐TK promoter in vitro. Five different proteins, LF‐A1, LF‐A2, LF‐B1, LF‐B2 and LF‐C, bind to the alpha 1AT promoter in liver extracts. LF‐A1 and LF‐B1 are positive transcriptional factors which bind to the A and B elements respectively. Their absence in spleen provides an explanation for the liver specificity of transcription. A protein similar to LF‐B2 is present in spleen. Binding of LF‐B1 and LF‐B2 to the alpha 1AT promoter is mutually exclusive, suggesting that LF‐B2 might be a repressor.