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MAS1, a gene essential for yeast mitochondrial assembly, encodes a subunit of the mitochondrial processing protease.
Author(s) -
Witte C.,
Jensen R. E.,
Yaffe M. P.,
Schatz G.
Publication year - 1988
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1988.tb02961.x
Subject(s) - biology , protein subunit , mitochondrial matrix , mitochondrion , hspa9 , protease , mutant , gene , microbiology and biotechnology , biochemistry , peptide sequence , enzyme , cytosol
We have previously described a yeast mutant (mas1) that accumulates mitochondrial precursor proteins at high temperature and is deficient in the activity of a matrix‐localized protease which cleaves presequences from mitochondrial precursor proteins. We have now cloned and sequenced the wild‐type MAS1 gene and found that it encodes a subunit of the mitochondrial processing protease, that it is essential for cell viability and that the protein product participates in its own cleavage during import into mitochondria. The MAS1 protein is thus the first genetically defined component of the mitochondrial protein import pathway.