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A gene‐specific promoter element is required for optimal expression of the histone H1 gene in S‐phase.
Author(s) -
Dalton S.,
Wells J. R.
Publication year - 1988
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1988.tb02782.x
Subject(s) - biology , gene , histone , transcription (linguistics) , gene expression , microbiology and biotechnology , promoter , transfection , genetics , linguistics , philosophy
An H1 gene‐specific element (H1‐box, 5′‐AAACACA‐3′) modulates S‐phase expression of the gene in vivo as judged by analysis of transcripts from histone genes transfected into HeLa cells. Deletion or base‐substitution of the element causes a 15‐ to 30‐fold decrease in steady‐state H1 mRNA levels in randomly growing cells and eliminates cell cycle control of transcription in synchronized cells. Mutations within the H1‐specific element which abolish S‐phase control of transcription also eliminate binding of a sequence‐specific nuclear factor capable of binding specifically to this region in vitro. Transfection of multiple copies of H1‐box elements into cells drastically decreases H1 mRNA levels, mimicking the effect observed when the motif is rendered non‐functional by deletion or substitution mutagenesis. In contrast, introduction of mutated H1 elements into cells has no detectable effect. Together, these results imply that an interaction between the H1‐box and a sequence‐specific trans‐acting factor modulates transcriptional control of H1 genes in vivo.