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Perforin is present only in normal activated Lyt2+ T lymphocytes and not in L3T4+ cells, but the serine protease granzyme A is made by both subsets.
Author(s) -
GarciaSanz J.A.,
Plaetinck G.,
Velotti F.,
Masson D.,
Tschopp J.,
MacDonald H.R.,
Nabholz M.
Publication year - 1987
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1987.tb04841.x
Subject(s) - biology , granzyme a , perforin , granzyme , serine protease , granzyme b , serine , microbiology and biotechnology , serine proteinase inhibitors , protease , proteases , cytotoxic t cell , t lymphocyte , virology , immunology , cd8 , biochemistry , immune system , enzyme , phosphorylation , in vitro
We show that among the subsets of peripheral T lymphocytes (Lyt2+ L3T4‐ and L3T4+ Lyt2‐ cells) activated in short‐term cultures by stimulation with H‐2 incompatible leukocytes 97% of the cytolytic activity and all detectable perforin activity resides in the Lyt2+ cells. But both populations contain approximately equal amounts of a serine protease, granzyme A, the expression of which was previously thought to be restricted to cytolytic T lymphocytes.