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A yeast mutant lacking mitochondrial porin is respiratory‐deficient, but can recover respiration with simultaneous accumulation of an 86‐kd extramitochondrial protein.
Author(s) -
Dihanich M.,
Suda K.,
Schatz G.
Publication year - 1987
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1987.tb04813.x
Subject(s) - porin , biology , mutant , yeast , mitochondrion , bacterial outer membrane , respiration , cytochrome c , glycerol , mitochondrial respiratory chain , inner mitochondrial membrane , biochemistry , gene , botany , escherichia coli
A yeast mutant lacking the only known pore‐forming protein of the mitochondrial outer membrane was constructed by gene disruption. The mutant retained all other major proteins of the mitochondrial outer membrane, but was severely deficient in mitochondrial cytochromes and initially did not grow on the non‐fermentable carbon source, glycerol. However, it could slowly adapt to glycerol; adaptation was accompanied by the partial restoration of cytochrome levels and massive accumulation of an 86‐kd polypeptide in extramitochondrial cell fractions.