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Fungal small nuclear ribonucleoproteins share properties with plant and vertebrate U‐snRNPs.
Author(s) -
Tollervey D.,
Mattaj I.W.
Publication year - 1987
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1987.tb04777.x
Subject(s) - snrnp , biology , ribonucleoprotein , small nuclear ribonucleoprotein , neurospora crassa , small nuclear rna , rna , guanosine , exoribonuclease , microbiology and biotechnology , genetics , non coding rna , rnase p , gene , mutant
snRNAs with properties closely related to those of the major vertebrate U‐snRNAs are present in the fungi Aspergillus nidulans, Neurospora crassa and Schizosaccharomyces pombe. These RNAs possess a tri‐methyl guanosine cap structure and a subset cross‐hybridizes with human U1 and U2 clones. In the form of snRNPs, snRNAs from these fungi as well as from Saccharomyces cerevisiae and pea plants are immunoprecipitated by human and anti‐Sm or anti‐(U1)RNP autoimmune antibodies. On micro‐injection into the cytoplasm of Xenopus oocytes, the snRNAs are packaged into ribonucleoprotein particles and migrate into the nucleus. The results demonstrate a hitherto unsuspected degree of evolutionary conservation in snRNA structure, snRNP protein structure, and sites of RNA‐protein interaction within snRNPs.