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A yeast small nuclear RNA is required for normal processing of pre‐ribosomal RNA.
Author(s) -
Tollervey D.
Publication year - 1987
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1987.tb02763.x
Subject(s) - ribosomal rna , biology , rna , rna processing , 5.8s ribosomal rna , saccharomyces cerevisiae , microbiology and biotechnology , ribosome , genetics , yeast , gene
In Saccharomyces cerevisiae, seven snRNAs (snR3, 4, 5, 8, 9, 10 and 17) are retained in the nucleus under conditions in which nucleoplasmic RNAs are lost, and may be nucleolar. All of these snRNAs show properties consistent with hydrogen bonding to pre‐ribosomal RNAs; snR5 and 8 with 20S pre‐rRNA, snR3, 4, 10 and 17 with 35S pre‐rRNA and snR9 with 20‐35S RNA. Strains lacking snR10 are impaired in growth and specifically defective in the processing of 35S RNA. Processing is slowed, leading to 35S RNA accumulation and most cleavage occurs, not at the normal sites, but at sites which in wild‐type strains are used for subsequent steps in rRNA maturation.