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Rolling circle replication of single‐stranded DNA plasmid pC194.
Author(s) -
Gros M. F.,
Riele H.,
Ehrlich S. D.
Publication year - 1987
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1987.tb02724.x
Subject(s) - biology , rolling circle replication , plasmid , replication (statistics) , genetics , dna , dna replication , computational biology , virology
A group of small Staphylococcus aureus/Bacillus subtilis plasmids was recently found to replicate via a circular single‐stranded DNA intermediate (te Riele et al., 1986a). We show here that a 55 bp region of one such plasmid, pC194, has origin activity when complemented in trans by the plasmid replication protein. This region contains two palindromes, 5 and 14 bp long, and a site nicked by the replication protein. DNA synthesis presumably initiated at the nick in the replication origin can be terminated at an 18 bp sequence homologous to the site of initiation, deriving from another plasmid, pUB110, or synthesized in vitro. This result suggests that, similar to the Escherichia coli single‐stranded DNA phages, pC194 replicates as a rolling circle. Interestingly, there is homology between replication origins and replication proteins of pC194 and the phage phi mX174.