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An ecdysone response element in the Drosophila hsp27 promoter
Author(s) -
Riddihough Guy,
Pelham Hugh R. B.
Publication year - 1987
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1987.tb02707.x
Subject(s) - ecdysone receptor , biology , ecdysone , microbiology and biotechnology , genetics , gene , nuclear receptor , transcription factor
It has previously been shown that a region of ˜100 bp in the Drosophila hsp27 promoter is sufficient to confer ecdysone inducibility on a heterologous gene. We now show, using binding and DNase I footprinting assays, that a 23‐bp hyphenated dyad within this sequence forms a protein‐binding site, and that this is sufficient for inducibility. The sequence shows partial homology with mammalian steroid receptor binding sites. UV crosslinking identifies an 80‐ to 90‐kd protein that binds specifically to this sequence and is thus a candidate for the ecdysone receptor.