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gal4 transcription activator protein of yeast can function as a repressor in Escherichia coli.
Author(s) -
Paulmier N.,
Yaniv M.,
WilckenBergmann B.,
MüllerHill B.
Publication year - 1987
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1987.tb02680.x
Subject(s) - biology , repressor , escherichia coli , yeast , activator (genetics) , transcription (linguistics) , escherichia coli proteins , saccharomyces cerevisiae , transcription factor , genetics , dna binding protein , microbiology and biotechnology , gene , linguistics , philosophy
The chromosomal lac operator of Escherichia coli was replaced by a 22 bp oligonucleotide containing the binding site of the yeast gal4 protein. Induction of gal4 protein synthesis in these bacteria repressed beta‐galactosidase synthesis at least 30‐fold. These results show that it is possible to detect in bacteria with a simple assay the DNA binding activity of a eukaryotic protein with a defined sequence specificity. This opens new avenues for the isolation in E. coli of mutants of DNA binding proteins unable to bind to their DNA targets, and for direct cloning in bacteria of cDNA coding for DNA binding proteins with defined sequence specificity.