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Biological function of ‘pathogenesis‐related’ proteins: four PR proteins of tobacco have 1,3‐β‐glucanase activity
Author(s) -
Kauffmann Serge,
Legrand Michel,
Geoffroy Pierrette,
Fritig Bernard
Publication year - 1987
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1987.tb02637.x
Subject(s) - humanities , biology , philosophy
Three of the ten acidic ‘pathogenesis‐related’ (PR) proteins known to accumulate in Nicotiana tabacum cv Samsun NN reacting hypersensitively to tobacco mosaic virus, namely −O, −N and −2, have been shown to have 1,3‐β‐glucanase (EC 3.2.1.39) activity. By using sera raised against each protein purified to homogeneity close serological relationships have been demonstrated between the three proteins. The same specific sera cross‐reacted with a basic protein which is also a 1,3‐β‐glucanase induced by virus infection and which can be considered as a new basic pathogenesis‐related protein of tobacco. Protein PR‐O and the basic 1,3‐β‐glucanase display about the same specific enzymatic activity, i.e. 50‐fold and 250‐fold higher than specific activities of proteins PR‐N and −2 respectively.