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Sps‐3 transcript levels are determined by multiple remote sequence elements.
Author(s) -
Giangrande A.,
Mettling C.,
Richards G.
Publication year - 1987
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1987.tb02615.x
Subject(s) - biology , enhancer , drosophila melanogaster , transcription (linguistics) , gene , genetics , drosophilidae , gene expression , microbiology and biotechnology , linguistics , philosophy
The region from 1.4 to 2.7 kb upstream of Drosophila melanogaster gene Sgs‐3 is responsible for a 10‐fold increase in Sgs‐3 transcript levels in the third instar larval salivary gland. This region includes the related Sgs‐7 gene from the 68C glue gene cluster as well as 400 bp of its 5′ sequences. We show that two elements are involved, each contributing a modest 3‐fold effect. One of these includes Sgs‐7 transcribed sequences some 2.3 kb upstream of Sgs‐3, although Sgs‐7 transcription is not involved. Although important for the overall levels of Sgs‐3 expression, they are clearly not strong, viral‐like enhancer elements. We propose that many position effects observed in P element transformation studies are the consequence of insertion in the vicinity of similar elements dispersed throughout the genome and having modest effects on transcript levels.