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CSF‐1‐induced gene expression in macrophages: dissociation from the mitogenic response.
Author(s) -
Orlofsky A.,
Stanley E. R.
Publication year - 1987
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1987.tb02599.x
Subject(s) - gene expression , cell culture , biology , immunology , gene , microbiology and biotechnology , genetics
Early gene expression associated with the mitogenic response to colony stimulating factor‐1 (CSF‐1) has been examined in BAC1.2F5, a CSF‐1‐dependent murine macrophage cell line. Stimulation of arrested cells by CSF‐1 resulted in acute, transient elevation in c‐fos and subsequently in c‐myc mRNA levels. Dramatic, sustained elevations were observed for JE and KC mRNAs, which are induced by platelet‐derived growth factor (PDGF) in 3T3 cells. The kinetics of expression of all four messages were similar to those reported in PDGF‐stimulated fibroblasts, implying a program of gene expression common to these two mitogens. Granulocyte‐macrophage CSF (GM‐CSF) can replace CSF‐1 in stimulating the growth of 2F5 cells. It induced mRNAs for c‐fos, c‐myc and JE but not KC. Therefore KC expression, although correlated with mitogenesis, is not required for proliferation. The effects of CSF‐1 were also examined in cells cycling continuously in its absence: 2F5 cells incubated in GM‐CSF and an autonomous variant subclone of 2F5. In either case, the only detected growth effect of CSF‐1 was a reduction in doubling‐time. Nevertheless, all four of the mRNAs induced by CSF‐1 in arrested cultures of 2F5 were strongly induced with the same kinetics in these cycling cells. Thus it would appear that the functions mediated by this early‐gene program are not restricted to the mitogenic stimulation of arrested cells.