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Structure and expression of human B cell stimulatory factor‐2 (BSF‐2/IL‐6) gene.
Author(s) -
Yasukawa K.,
Hirano T.,
Watanabe Y.,
Muratani K.,
Matsuda T.,
Nakai S.,
Kishimoto T.
Publication year - 1987
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1987.tb02598.x
Subject(s) - biology , gene expression , gene , microbiology and biotechnology , regulation of gene expression , genetics
The chromosomal DNA segment of human B cell stimulatory factor‐2 (BSF‐2/IL‐6) was isolated and characterized by nucleotide sequence analysis. The human BSF‐2/IL‐6 gene consists of five exons and four introns and its organization shows a distinctive similarity to granulocyte colony‐stimulating factor gene. The two genes have the same number of exons and introns and the size of each exon is strikingly similar. The BSF‐2/IL‐6 mRNA was found to be constitutively expressed in a human T cell leukemia virus‐1 transformed T cell line, TCL‐Na1, a bladder cell carcinoma line, T24, and an amnion derived cell line, FL. The BSF‐2/IL‐6 mRNA was also found to be inducible with interleukin‐1 beta in an astrocytoma line, U373 and a glioblastoma line, SK‐MG‐4. S1 mapping and primer extension analyses showed the presence of multiple initiation sites and the preferential utilization of a different initiation site for each individual tissue tested.