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The 5′‐proximal region of the wheat Cab‐1 gene contains a 268‐bp enhancer‐like sequence for phytochrome response.
Author(s) -
Nagy F.,
Boutry M.,
Hsu M. Y.,
Wong M.,
Chua N. H.
Publication year - 1987
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1987.tb02541.x
Subject(s) - plant biology , biology , library science , computational biology , botany , computer science
We have previously reported that the expression of the wheat Cab‐1 gene is subject to phytochrome regulation and a 1.8‐kb 5′ upstream sequence of this gene is sufficient for the regulated expression. To delineate sequences for the phytochrome response we analyzed a series of 5′ deletion mutants as well as chimeric gene constructs comprising different sequences of the Cab‐1 upstream region in transgenic tobacco seedlings. We found that a deletion mutant containing a 357‐bp 5′ upstream sequence still exhibits maximal levels of phytochrome‐regulated expression. A 268‐bp enhancer‐like element, located between −89 and −357, is responsible for the phytochrome response of the Cab‐1 gene; sequences upstream from −357 to −843 and downstream from −124 to +1100 are probably not involved. Finally, we show that the Cab‐1 mRNA stability is not regulated by phytochrome.