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Amino acid sequence of the cytochrome subunit of the photosynthetic reaction centre from the purple bacterium Rhodopseudomonas viridis
Author(s) -
Weyer K. A.,
Lottspeich F.,
Gruenberg H.,
Lang F.,
Oesterhelt D.,
Michel H.
Publication year - 1987
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1987.tb02490.x
Subject(s) - biology , rhodospirillaceae , rhodospirillales , rhodopseudomonas , cytochrome , protein subunit , rhodopseudomonas palustris , bacteria , peptide sequence , biochemistry , botany , amino acid , microbiology and biotechnology , photosynthesis , enzyme , genetics , gene
The complete nucleotide sequence of the gene encoding the cytochrome subunit of the photosynthetic reaction centre from the purple bacterium Rhodopseudomonas viridis , and the derived amino acid sequence are presented. The nucleotide sequence of the gene reveals the existence of a typical bacterial signal peptide of 20 amino acid residues which is not found in the mature cytochrome subunit. The gene encoding the cytochrome subunit is preceded by the gene encoding the M subunit. Both genes overlap by 1 bp. The mature cytochrome subunit consists of 336 amino acid residues; 73% of its amino acid sequence was confirmed by protein sequencing work. The mol. wt of the cytochrome subunit including the covalently bound fatty acids and the bound heme groups is 40 500. The internal sequence homology is low, despite the symmetric structure of the cytochrome subunit previously shown by X‐ray crystallographic analysis of the intact photosynthetic reaction centre. Sequence homologies to other cytochromes were not found.