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The yeast homologue of U3 snRNA.
Author(s) -
Hughes J. M.,
Konings D. A.,
Cesareni G.
Publication year - 1987
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1987.tb02482.x
Subject(s) - biology , small nuclear rna , genetics , yeast , prp24 , snrnp , saccharomyces cerevisiae , microbiology and biotechnology , rna splicing , rna , gene , non coding rna
snR17, one of the most abundant capped small nuclear RNAs of Saccharomyces cerevisiae, is equivalent to U3 snRNA of other eukaryotes. It is 328 nucleotides in length, 1.5 times as long as other U3 RNAs, but shares significant homology both in nucleotide sequence and in predicted secondary structure. Human scleroderma antiserum specific to nucleolar U3 RNP can enrich snR17 from sonicated yeast nuclear extracts. Unlike other yeast snRNAs which are encoded by single copy genes, snR17 is encoded by two genetically unlinked genes: SNR17A and SNR17B. The RNA snR17A is more abundant than snR17B. Deleting one or other of the genes has no obvious phenotypic effect, except that the steady‐state level of snR17B is increased in snr17a‐ strains. Haploid strains with both genes deleted are inviable, therefore yeast U3 is essential.