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Human papillomavirus type 16 DNA cooperates with activated ras in transforming primary cells.
Author(s) -
Matlashewski G.,
Schneider J.,
Banks L.,
Jones N.,
Murray A.,
Crawford L.
Publication year - 1987
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1987.tb02426.x
Subject(s) - biology , dna , human papillomavirus , primary (astronomy) , virology , genetics , bovine papillomavirus , cancer research , microbiology and biotechnology , gene , genome , medicine , physics , astronomy
The close association of human papillomavirus type 16 DNA with a majority of cervical carcinomas implies some role for the virus in this type of cancer. To define the transforming properties of HPV‐16 DNA in vitro we have now performed transfection experiments on baby rat kidney cells using HPV‐16 DNA in conjunction with an activated ras gene. We have demonstrated that a 6.6‐kb DNA fragment, containing the early genes of HPV‐16 under the control of Moloney murine leukaemia virus long terminal repeats (MoMuLV‐LTRs), cooperates with EJ‐ras in transforming these cells. Both DNAs are required and neither alone is effective. The cooperating activity appears to reside in a protein or proteins derived from the E6/E7 region of the HPV‐16 genome.