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The activation antigen BLAST‐2, when shed, is an autocrine BCGF for normal and transformed B cells.
Author(s) -
Swendeman S.,
ThorleyLawson D. A.
Publication year - 1987
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1987.tb02412.x
Subject(s) - autocrine signalling , biology , lymphoblast , epstein–barr virus , antigen , lymphokine , microbiology and biotechnology , b cell , t cell , immune system , receptor , virus , immunology , cell culture , antibody , genetics
A shed form of the membrane‐bound B cell activation marker, BLAST‐2 (Epstein‐Barr virus cell surface, CD 23) was immune‐affinity purified from Epstein‐Barr virus‐transformed lymphoblast conditioned medium. SDS‐PAGE analysis revealed a complex of two polypeptides, mol. wts 25,000 and 12,000, here termed s‐BLAST‐2. We show that this complex, when purified to homogeneity, can act as a growth factor for EBV‐infected B lymphoblasts and normal receptor‐stimulated B cell blasts. It has no effect on resting B or T cells. These data suggest that the BLAST‐2 antigen has a role in autocrine B cell growth. Additionally, this complex is a co‐mitogen for PHA‐stimulated murine thymocytes, a property of interleukin‐1.