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DNA recognition by a new family of type I restriction enzymes: a unique relationship between two different DNA specificities.
Author(s) -
Price C.,
Shepherd J. C.,
Bickle T. A.
Publication year - 1987
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1987.tb02391.x
Subject(s) - restriction enzyme , biology , dna , type (biology) , genetics , recognition sequence , dna sequencing , enzyme , biochemistry , ecology
The DNA sequences recognized by the allelic type I restriction enzymes EcoR124 and EcoR124/3 were determined. EcoR124 recognizes 5′‐GAA(N6)RTCG‐3′ and EcoR124/3 recognizes 5′‐GAA(N7)RTCG‐3′. These are typical of sequences recognized by type I recognition enzymes in that they consist of two specific domains separated by a non‐specific spacer sequence. For these two enzymes, the specific sequences are identical but the length of the non‐specific spacer is different. The specific domains of EcoR124/3 are thus 3.4 A further apart than those of EcoR124 and rotated with respect to each other through a further 36 degrees.