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Localization of the mcf.2 transforming sequence to the X chromosome.
Author(s) -
Noguchi T.,
Mattei M. G.,
Oberlè I.,
Planche J.,
Imbert J.,
Pelassy C.,
Birg F.,
Birnbaum D.
Publication year - 1987
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1987.tb02368.x
Subject(s) - sequence (biology) , library science , biology , computer science , genetics
A transforming sequence was identified using co‐transfection of DNA from the human mammary carcinoma cell line MCF‐7 and of a G418 resistance gene into NIH 3T3 cells, followed by tumor formation in athymic mice. This sequence, named mcf.2, was molecularly cloned. A transforming activity resides in a cosmid clone of 42 kb. mcf.2 did not cross‐hybridize with the known oncogenes tested. In situ hybridization localized it on the X chromosome, probably at q27. This localization was confirmed by hybridization to a panel of human–rodent cell line DNAs.