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Homology between the ran1+ gene of fission yeast and protein kinases.
Author(s) -
McLeod M.,
Beach D.
Publication year - 1986
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1986.tb04697.x
Subject(s) - biology , homology (biology) , genetics , gene , yeast , kinase , fission , schizosaccharomyces , protein serine threonine kinases , sequence homology , schizosaccharomyces pombe , fungal protein , saccharomyces cerevisiae , computational biology , peptide sequence , protein kinase a , physics , quantum mechanics , neutron
The ran1+ gene of the fission yeast Schizosaccharomyces pombe is a negative regulator of both sexual conjugation and meiosis. The nucleotide sequence of the gene has been determined and contains a region of open reading frame (ORF) capable of encoding a protein of 52,000 daltons. S1 nuclease analysis of ran1+‐encoded RNA showed that the ORF was spanned by an uninterrupted transcript. A fragment of DNA containing the entire ran1+ gene was expressed in a bacterial expression vector and found to encode the expected product of 52,000 daltons. The putative ran1+ gene product shares significant sequence homology with known protein kinases. The level of the ran1+ transcript was similar in vegetative and meiotic cells suggesting that the ran1+ protein product rather than its transcript is regulated during sexual differentiation.