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Two Drosophila chorion genes terminate transcription in discrete regions near their poly(A) sites.
Author(s) -
Osheim Y.N.,
Miller O.L.,
Beyer A.L.
Publication year - 1986
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1986.tb04687.x
Subject(s) - biology , transcription (linguistics) , gene , drosophila melanogaster , miller , chromatin , nucleotide , genetics , microbiology and biotechnology , philosophy , linguistics , ecology
We have examined transcription termination of two closely linked Drosophila melanogaster chorion genes, s36‐1 and s38‐1, using the electron microscope. Our method is unusual and is independent of in vitro nuclear run‐on transcription. By measuring transcription unit lengths in chromatin spreads, we can localize efficient termination sites to a region of approximately 210 bp for s36‐1 and approximately 365 bp for s38‐1. The center of this region is approximately 105 nucleotides downstream of the poly(A) site for the s36‐1 gene, and approximately 400 nucleotides downstream for the s38‐1 gene. Thus, these two Drosophila chorion genes terminate more closely to their poly(A) addition sites and in a shorter region than many other polyadenylated genes examined to date.