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The sequence of carnation etched ring virus DNA: comparison with cauliflower mosaic virus and retroviruses
Author(s) -
Hull R.,
Sadler J.,
Longstaff M.
Publication year - 1986
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1986.tb04614.x
Subject(s) - biology , cauliflower mosaic virus , virology , virus , dna , sequence (biology) , mosaic virus , genetics , plant virus , gene , transgene , genetically modified crops
Carnation etched ring virus (CERV) DNA comprises 7932 bp. CERV primer binding sites and overall genome organization are similar to those of the related cauliflower mosaic virus (CaMV). The six open reading frames of CERV showed amino acid homology (50‐80%) with CaMV ORFs I‐VI; no homologues of CaMV ORFs VII or VIII were found. CERV ORFs 1‐5 interface each other with the sequence ATGA. The comparison of CERV ORF5 with CaMV ORFV highlighted regions which show homologies to retrovirus gag/pol protease, RNase H and DNA polymerase domains; the possibility that the DNA polymerase domain comprises two subdomains, operating off different templates, is discussed. Both CERV and CaMV ORFs I have sequence homology to tobacco mosaic virus P30 and plastocyanin.