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Monocot and dicot pre‐mRNAs are processed with different efficiencies in transgenic tobacco
Author(s) -
Keith Brian,
Chua NamHai
Publication year - 1986
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1986.tb04516.x
Subject(s) - biology , polyadenylation , gene , transgene , intron , nicotiana tabacum , genetically modified crops , promoter , genetics , transcription (linguistics) , regulatory sequence , rna , rubisco , gene expression , linguistics , philosophy
A gene encoding the small subunit of ribulose 1,5‐bisphosphate carboxylase ( rbcS ) in wheat, a monocot plant, was transferred to tobacco, a dicot plant. The wheat gene is not expressed in transgenic tobacco under the control of its own promoter, but when transcription is driven by a viral promoter, several wheat transcripts accumulate. These include both spliced and unspliced transcripts, which are polyadenylated at multiple novel sites in the wheat 3′ flanking region. Another monocot intron, from the maize Adh‐1 gene, is also spliced inefficiently in tobacco. These findings contrast results demonstrating efficient processing of different rbcS transcripts from pea, a dicot, in transgenic tobacco. This pattern may reflect general differences in sequences required for RNA processing in monocot and dicot plants.