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Nuclear location of all three influenza polymerase proteins and a nuclear signal in polymerase PB2.
Author(s) -
Jones I.M.,
Reay P.A.,
Philpott K.L.
Publication year - 1986
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1986.tb04506.x
Subject(s) - biology , polymerase , microbiology and biotechnology , virology , gene , genetics
In order to re‐examine the sub‐cellular location of the three influenza A/NT/60/68 polymerase proteins PB1, PB2 and PA in infected cells, specific antisera for each polymerase component have been prepared by immunizing rabbits with polymerase‐beta‐galactosidase fusion proteins synthesized in Escherichia coli. We show that polymerase PB1, PB2, and PA are predominantly associated with the nucleus of influenza‐infected MDCK cells by immunocytochemical techniques. In the case of polymerase PB2 we investigate the possibility that nuclear accumulation is an intrinsic property of the PB2 protein. Using a vaccinia‐PB2 recombinant virus, we show that PB2 accumulates intra‐nuclearly in monkey CV‐1 cells in the absence of any other influenza protein, suggesting it contains an intrinsic nuclear signal.