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Cis‐acting sequences which regulate expression of the Sgs‐4 glue protein gene of Drosophila.
Author(s) -
McNabb S.L.,
Beckendorf S.K.
Publication year - 1986
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1986.tb04501.x
Subject(s) - biology , gene , drosophilidae , mutant , gene expression , genetics , hypersensitive site , regulatory sequence , regulation of gene expression , microbiology and biotechnology , drosophila melanogaster , enhancer
The Sgs‐4 glue protein gene of Drosophila is expressed only in third‐instar larval salivary glands. Previous work suggests that a regulatory region lies 5′ and remote to the gene, as indicated by a region of tissue‐specific DNase I hypersensitivity and by underproducing mutants with DNA lesions in the hypersensitive region. Here we demonstrate by germ line transformation of cloned fragments containing Sgs‐4 that the sequences between 840 bp 5′ and 130 bp 3′ to the gene are sufficient for Sgs‐4 activity. When 5′ sequence was removed to ‐392, activity was eliminated, thereby verifying the existence of essential sequences far upstream. Fragments that are active include, in addition to the capacity for normal levels of expression, three other cis‐acting regulatory activities: developmental timing, tissue specificity, and dosage compensation. In contrast, the fragments tested did not specify formation of the puff with which Sgs‐4 is normally associated. As shown by chromosomal rearrangements, the region required for puffing is limited to 16‐19 kb surrounding the gene.